If so, the program determines the locations of the start and stop codons. The length of the protein, the presence of the Kozak consensus sequence at the start codon and the length of the reference predicted protein are weighed by the program as positive. From the research, the results show that the ANKRD42 gene was found to exhibit bright bands of the expected size. Ciliated cells exhibited strong immunoreactivity in cilia, neuronal cells were moderately stained while other normal cells exhibited weak stains or negative expression. The gene is well expressed with the expression being detected in normal cells including the testis, brain cerebellum, fetal brain, heart, Rathke’s pouches, cecum, urinary bladder, spinal cord, prostate, salivary gland, thymus, skeletal muscle and small intestine among other tissues. Due to this, it was put aside and no further tests were conducted to investigate its potential as a cancer marker. MAEL Gene The MAEL gene is described as maelstrom spermatogenic transposon silencer which creates a protein that was initially located in Drosophila melanogaster in the nuage perinuclear structure. It is believed to have a functionality that is similar to the spindle gene class. The gene Maelstrom is a protein-coding gene that is associated with diseases such as gonorrhea and essential hypertension. The gene encodes a novel protein that is distributed in the cytoplasm of the nurse cell as well as the oocyte until the protein disappears in stage 7 of oogenesis.