Process of protein synthesis and mutation

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What could be the base sequence of the RNA after transcription happens? Explain the importance of first and last codons? Specify the meaning do these codons have for the protein synthesis? For each of two, you will require to first transcribe the mRNA, and then utilize the genetic code table to find out the amino acid sequence.

Question: Explain why modifications in amino acid sequence might affect protein function.

Below is the original sequence, followed by the two mutated sequences, 1 and 2:

Original sequence 3′- TACCCTTTAGTAGCCACT-5′

Mutated sequence 1) 3′-TACGCTTTAGTAGCCATT-5′

Mutated sequence 2) 3′-TAACCTTTACTAGGCACT-5′.

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