you decide that you want to figure out a situation where you see constant transcription of the lac genes but you want the amount of transcription to be low. which scenarios will give low transcription?
1. make a loss of function mutation in the operator of the lac operon and aID glucose
2. make a loss of function mutation in CAP and aID lactose
3. Make a mutation in the promoter of the LacI repressor and aID glucose and lactose
4. make a mutation in the LacI repressor such that it cannot bind inducer but can still bind the DNA
5. make a loss of function mutation in the LacY gene