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Insolating different species of bacteria

After insolating different species of bacteria, you see two distinct types of colonies. One has a medium-sized shimy colonies that are mucoid with entire margins, convex elevation and a whitish traslucent appearance. This colony type is the dominant type on the plate. The othe has circular, pinhead colonies which are convex with entire margins and have a yellowish-while color. There are only 2 olonies that appear this way.
You make pure culture of each type of colony using half of one colony, labeled with its colony description, You take the other half of thew colony you used to make your pure culture, and perform a simple stain to determine the size and shape of the different bacteria.
1)What kind of simple stain would you perform and why? What color will the cells appear when looking through the microscope and why?

2) Do you think the cells’ shape has been affected by the stain? Why or why not?

3)You determine that the cells from the whitish translucent colony are rod shaped. What term do you use to describe this cell type? ____________
4) You find that the cells from the smaller, yellowish-white colonies are circular and seem to appear in clumps. What cell type is this? ________________
5) After you incubate your pure culture for 24 hours, you perform a gram stain. You find that your circular cells have stained purple. What does this result mean? Don’t just answer gram+ or gram-. Give a brief explanation what this reaction is based on!

6) You find that your rod shaped cells have stained pink. What does this result mean? Don’t just answer gram+ or gram-. Give a brief explanation what this reaction is based on!




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