Techniques and Procedures – The graduate uses appropriate techniques or procedures for interacting with microorganisms in a clinic, laboratory, or community setting; utilizes basic laboratory techniques for identifying microorganisms; demonstrates proper laboratory techniques in microbiology, including sterile technique, staining techniques, aseptic technique, and chemical indicators; and recognizes types of culture media and when to use each.

-Differentiate between specified types of bacteria-caused disease.

-Use a specified differential staining technique in a laboratory setting.

Write a brief essay (suggested length of 1-2 pages) in which you:
A. Describe the differences between gram-positive and gram-negative cell walls.
B. Explain what causes gram-negative bacteria to stain pink.
C. Explain what causes gram-positive bacteria to stain purple.
D. Explain the purpose of iodine in the Gram stain procedure.

1. Define what a mordant is.
E. Explain the purpose of acetone-alcohol in the Gram stain procedure.
F. Identify which organisms stained gram-positive.

1. Identify an infection most commonly associated with each identified organism (the organism can be probiotic, not necessarily the cause of the infection).
G. Identify which organisms stained gram-negative.

1. Identify an infection most commonly associated with each identified organism (the organism can be probiotic, not necessarily the cause of the infection).
H. When you use sources, include all in-text citations and references in APA format.