In the naturally occurring variant hemoglobin Rainier, Tyr145? is replaced with a Cys. This mutation allows for disulfide bond formation with a nearby Cys in the same subunit.
a. By examining the structure of hemoglobin (Pdb file = 1HGA), suggestwhich Cys makes this new disulfide.
b. Formation of this disulfide prevents the formation of some of theion pairs found exclusively in the T-state. Does hemoglobin Rainierbind oxygen more or less tightly than normal hemoglobin?
c. Does hemoglobin Rainier bind have a greater or lesser Bohr effectthan normal hemoglobin.
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