You are asked todetermine the concentration of a bacterial culture. You take 1.0 mLof the culture and aID it to 4.0 mL of water (Tube #1). You thentake 1.0 mL from Tube #1 and aID it to 14 mL of water (Tube #2).Finally, you take 1.0 mL from Tube #2 and aID it to 19 mL of water(Tube #3). You then spread 0.20 mL from Tube #3 onto a nutrientagar plate and incubate the plate at 37°C. After 24 hours, youcount 215 colonies on the plate.
A. By what factor has the original culture beendiluted in Tube #1? B. By what factor has the ORIGINAL culturebeen diluted in Tube #2? C. By what factor has the ORIGINAL culturebeen diluted in Tube #3? D. What is the concentration of the original bacterial culture?
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