Separate the denatured protein into several components

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Suppose you suspect that there is more than one type of subunit in the native molecule. So you treat the native molecule with mercaptoethanol and urea and try to separate the denatured protein into several components. If there really are 2 (or more) different types of subunits, which of the following methods is most likely to separate them? (dialysis) (gel filtration) (paper chromatography) (column chromatography based on solubility) (gel electrophoresis) (hydrolysis) (any of these should work)

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