Describe briefly orthogonal field alternation gel electrophoresis.
1. Distinguish between a nick and a discontinuity in DNA and name the enzymes that repair them. (3 marks)2. Outline the steps involved in the use horseradish peroxidase in detection of DNA of interest in an agarose gel.3. State six applications of PCR in clinical diagnosis4. Outline the steps involved in the use of (enzyme-linked …
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